Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 85
Page 8-27
... allow the sequences at the 5 ' terminus of the mRNA ( which are often lost during conventional cloning procedures ) to be cloned . Its major disadvantage , however , is that it is at least ten times less efficient than double - stranded ...
... allow the sequences at the 5 ' terminus of the mRNA ( which are often lost during conventional cloning procedures ) to be cloned . Its major disadvantage , however , is that it is at least ten times less efficient than double - stranded ...
Page 8-70
... allow the cDNA to enter the gel matrix . Wash the microfuge tube used to store the cDNA with 50 μl of TE ( pH 7.6 ) , and apply this to the column . Fill the bubble tubing with TE ( pH 7.6 ) containing 0.1 M NaCl . Important : Do not allow ...
... allow the cDNA to enter the gel matrix . Wash the microfuge tube used to store the cDNA with 50 μl of TE ( pH 7.6 ) , and apply this to the column . Fill the bubble tubing with TE ( pH 7.6 ) containing 0.1 M NaCl . Important : Do not allow ...
Page 9-22
... Allow the powders to spread over the surface of the lysis solution , and then shake the beakers to submerge the material . When all the material is in solution , transfer the solutions to centrifuge tubes . 3. Close the tops of the ...
... Allow the powders to spread over the surface of the lysis solution , and then shake the beakers to submerge the material . When all the material is in solution , transfer the solutions to centrifuge tubes . 3. Close the tops of the ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector