Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 87
Page 8-73
... amount of bacteriophage A arms is ligated to varying amounts of cDNA . The aim is to determine the amount of cDNA that yields at least 5 × 106 recombinant bacteriophages ( i.e. , a mammalian cDNA library of reasonable size ) . The ...
... amount of bacteriophage A arms is ligated to varying amounts of cDNA . The aim is to determine the amount of cDNA that yields at least 5 × 106 recombinant bacteriophages ( i.e. , a mammalian cDNA library of reasonable size ) . The ...
Page 10-9
... amount of the unlabeled dNTP . Most commercial suppliers sell [ a - 32P ] dNTPs in a concentrated aqueous solution ... amount of DNAase I in the reaction . The aim is to establish conditions that will result in incorporation of about 30 ...
... amount of the unlabeled dNTP . Most commercial suppliers sell [ a - 32P ] dNTPs in a concentrated aqueous solution ... amount of DNAase I in the reaction . The aim is to establish conditions that will result in incorporation of about 30 ...
Page 14-31
... amount of radioactivity in them in a liquid scintillation counter . Calculate the relative amounts of the two radiolabeled DNAs in each of the polymer- ase chain reactions . Remember to correct the amount of radioactivity to allow for ...
... amount of radioactivity in them in a liquid scintillation counter . Calculate the relative amounts of the two radiolabeled DNAs in each of the polymer- ase chain reactions . Remember to correct the amount of radioactivity to allow for ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector