Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 14-5
A Laboratory Manual Joseph Sambrook, Tom Maniatis. APPLICATIONS OF PCR AMPLIFICATION Although a relatively new technique , PCR amplification has already found extensive application in the diagnosis of genetic disorders ( Wong et al ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. APPLICATIONS OF PCR AMPLIFICATION Although a relatively new technique , PCR amplification has already found extensive application in the diagnosis of genetic disorders ( Wong et al ...
Page 14-28
... Amplification In this method ( Gyllensten and Erlich 1988 ; Mihovilovic and Lee 1989 ) , amplification reactions are set up that contain vastly different molar ratios of the two oligonucleotide primers . During the first 10-15 cycles of ...
... Amplification In this method ( Gyllensten and Erlich 1988 ; Mihovilovic and Lee 1989 ) , amplification reactions are set up that contain vastly different molar ratios of the two oligonucleotide primers . During the first 10-15 cycles of ...
Page 14-30
... amplify and detect minute quantities of nucleic acids , it has not been used routinely to quantitate the concentration of the sequences of interest in the original sample of nucleic acids . This is because the efficiency of the ...
... amplify and detect minute quantities of nucleic acids , it has not been used routinely to quantitate the concentration of the sequences of interest in the original sample of nucleic acids . This is because the efficiency of the ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml