Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 12-11
... antigen ( 20 pg to 100 ng ) of interest are spotted onto a separate filter . The three filters are then cut into sections , which are screened as described below with different dilutions of antiserum . The aim is to find a dilution that ...
... antigen ( 20 pg to 100 ng ) of interest are spotted onto a separate filter . The three filters are then cut into sections , which are screened as described below with different dilutions of antiserum . The aim is to find a dilution that ...
Page 12-13
... antigen - specific purifi- cation is not necessary . However , if the antibodies that react with the antigen of interest are of the IgG class , they may be easily purified and concentrated by affinity chromatography on protein A ...
... antigen - specific purifi- cation is not necessary . However , if the antibodies that react with the antigen of interest are of the IgG class , they may be easily purified and concentrated by affinity chromatography on protein A ...
Page 12-14
... antigen on nitrocellulose filters are : • 125I - labeled antibodies reactive with species - specific determinants on the primary antibodies ( Helfman et al . 1983 ) . • Protein A of Staphylococcus aureus radiolabeled with 125I ( Kemp ...
... antigen on nitrocellulose filters are : • 125I - labeled antibodies reactive with species - specific determinants on the primary antibodies ( Helfman et al . 1983 ) . • Protein A of Staphylococcus aureus radiolabeled with 125I ( Kemp ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml