Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 78
Page 9-29
... appropriate size . 2. In a sterile microfuge tube , mix : partially digested genomic DNA 10 x Sau3AI restriction enzyme buffer 1 mм dATP 20 με 5 μ . 2 μ . 1 mм dGTP 2 μ . H2O to 46 μl Klenow fragment of E. coli DNA polymerase I ( 5 ...
... appropriate size . 2. In a sterile microfuge tube , mix : partially digested genomic DNA 10 x Sau3AI restriction enzyme buffer 1 mм dATP 20 με 5 μ . 2 μ . 1 mм dGTP 2 μ . H2O to 46 μl Klenow fragment of E. coli DNA polymerase I ( 5 ...
Page 15-25
... appropriate 10 x restriction enzyme buffer and 8 units of a restriction enzyme that will separate the target DNA from the vector . Incubate the reactions for 1 hour at the appropriate temperature . 13. At the end of the incubation ...
... appropriate 10 x restriction enzyme buffer and 8 units of a restriction enzyme that will separate the target DNA from the vector . Incubate the reactions for 1 hour at the appropriate temperature . 13. At the end of the incubation ...
Page 15-30
... appropriate 10 × restriction enzyme buffer the appropriate restriction enzyme 22 μ . 5 με 5-10 units Incubate the reaction for 2 hours at the appropriate temperature . 16. Transform ( plasmids or phagemids ) or transfect ( bacteriophage ...
... appropriate 10 × restriction enzyme buffer the appropriate restriction enzyme 22 μ . 5 με 5-10 units Incubate the reaction for 2 hours at the appropriate temperature . 16. Transform ( plasmids or phagemids ) or transfect ( bacteriophage ...
Other editions - View all
Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml