Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-18
... aqueous phases . 5. With a wide - bore pipette ( 0.3 - cm - diameter orifice ) , transfer the viscous aqueous phase to a clean centrifuge tube and repeat the extraction with phenol twice . When transferring the aqueous phase , it is ...
... aqueous phases . 5. With a wide - bore pipette ( 0.3 - cm - diameter orifice ) , transfer the viscous aqueous phase to a clean centrifuge tube and repeat the extraction with phenol twice . When transferring the aqueous phase , it is ...
Page 9-47
... aqueous solution . However , it has been found that the kinetics of hybridization in 80 % formamide are approximately four times slower than in aqueous solution ( Casey and Davidson 1977 ) . Assuming a linear relationship between the ...
... aqueous solution . However , it has been found that the kinetics of hybridization in 80 % formamide are approximately four times slower than in aqueous solution ( Casey and Davidson 1977 ) . Assuming a linear relationship between the ...
Page 12-39
... aqueous solutions . The rate of inactivation increases with pH and is faster at 25 ° C than at 4 ° C . The half - life of a 20 μM aqueous solution of PMSF is about 35 minutes at pH 8.0 ( James 1978 ) . This means that aqueous solutions ...
... aqueous solutions . The rate of inactivation increases with pH and is faster at 25 ° C than at 4 ° C . The half - life of a 20 μM aqueous solution of PMSF is about 35 minutes at pH 8.0 ( James 1978 ) . This means that aqueous solutions ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml