Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 25
Page xxvii
... Assays for Chloramphenicol Acetyltransferase 16.60 METHOD 1 : THIN - LAYER CHROMATOGRAPHY 16.60 METHOD 2 : EXTRACTION WITH ORGANIC SOLVENTS 16.63 METHOD 3 : DIFFUSION OF REACTION PRODUCTS INTO SCINTILLATION FLUID 16.64 Assay for B ...
... Assays for Chloramphenicol Acetyltransferase 16.60 METHOD 1 : THIN - LAYER CHROMATOGRAPHY 16.60 METHOD 2 : EXTRACTION WITH ORGANIC SOLVENTS 16.63 METHOD 3 : DIFFUSION OF REACTION PRODUCTS INTO SCINTILLATION FLUID 16.64 Assay for B ...
Page 12-32
... assays are used to measure the activity of bacteriophage T4 DNA ligase . Most manufacturers ( apart from New England ... assay ( Modrich and Lehman 1970 ) and to 60 cohesive - end units ( as defined by New England Biolabs ) . 0.015 Weiss ...
... assays are used to measure the activity of bacteriophage T4 DNA ligase . Most manufacturers ( apart from New England ... assay ( Modrich and Lehman 1970 ) and to 60 cohesive - end units ( as defined by New England Biolabs ) . 0.015 Weiss ...
Page 15-30
... assays are used to measure the activity of bacteriophage T4 DNA ligase . Most manufacturers ( apart from New England ... assay ( Modrich and Lehman 1970 ) and to 60 cohesive - end units ( as defined by New England Biolabs ) . 0.015 Weiss ...
... assays are used to measure the activity of bacteriophage T4 DNA ligase . Most manufacturers ( apart from New England ... assay ( Modrich and Lehman 1970 ) and to 60 cohesive - end units ( as defined by New England Biolabs ) . 0.015 Weiss ...
Other editions - View all
Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml