Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-74
... bacteria of both strains as described in Chapter 2 , page 2.60 . ( It may be necessary to suspend the bacteria of strain BNN102 in a smaller volume of 10 mM MgSO , than usual to attain a density of 1.5 x 10 cells / ml . ) - Measure the ...
... bacteria of both strains as described in Chapter 2 , page 2.60 . ( It may be necessary to suspend the bacteria of strain BNN102 in a smaller volume of 10 mM MgSO , than usual to attain a density of 1.5 x 10 cells / ml . ) - Measure the ...
Page 9-30
... bacteria . Incubate for 20 minutes at 37 ° C . 2. Mix 6.5 ml of melted top agar or agarose with each aliquot of infected bacteria and spread on a freshly poured 150 - mm plate of bottom agar . Alternatively , as many as 450,000 ...
... bacteria . Incubate for 20 minutes at 37 ° C . 2. Mix 6.5 ml of melted top agar or agarose with each aliquot of infected bacteria and spread on a freshly poured 150 - mm plate of bottom agar . Alternatively , as many as 450,000 ...
Page 12-21
... bacteria are plated directly from a transformation mixture onto detergent- free nitrocellulose filters ( Millipore ... bacteria in a small volume of liquid ( < 0.5 ml containing up to 20,000 bacteria for a 138 - mm filter ; < 0.2 ml ...
... bacteria are plated directly from a transformation mixture onto detergent- free nitrocellulose filters ( Millipore ... bacteria in a small volume of liquid ( < 0.5 ml containing up to 20,000 bacteria for a 138 - mm filter ; < 0.2 ml ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml