Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxx
... BACTERIAL CULTURES GROWING IN LIQUID MEDIA A.5 BACTERIAL CULTURES GROWING ON AGAR PLATES A.5 ANTIBIOTICS A.6 SOLUTIONS FOR WORKING WITH BACTERIOPHAGEA.7 Maltose A.7 SM A.7 TM A.7 A Diluent A.7 BACTERIAL STRAIN LIST A.9 Appendix B ...
... BACTERIAL CULTURES GROWING IN LIQUID MEDIA A.5 BACTERIAL CULTURES GROWING ON AGAR PLATES A.5 ANTIBIOTICS A.6 SOLUTIONS FOR WORKING WITH BACTERIOPHAGEA.7 Maltose A.7 SM A.7 TM A.7 A Diluent A.7 BACTERIAL STRAIN LIST A.9 Appendix B ...
Page 12-22
... bacterial colonies Whatman 3MM papers 0 replica - plating tool sterile velvet replica nitrocellulose filter template nitrocellulose filter carrying bacterial colonies hard surface FIGURE 12.1 Use of a replica - plating tool to replicate ...
... bacterial colonies Whatman 3MM papers 0 replica - plating tool sterile velvet replica nitrocellulose filter template nitrocellulose filter carrying bacterial colonies hard surface FIGURE 12.1 Use of a replica - plating tool to replicate ...
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... bacterial plates for a few hours at 37 ° C to allow the colonies to regrow , and then store the plates at 4 ° C until they are needed . Lyse the colonies transferred to the filter with alkali ( see Chapter 1 ) . Neutralize the filters ...
... bacterial plates for a few hours at 37 ° C to allow the colonies to regrow , and then store the plates at 4 ° C until they are needed . Lyse the colonies transferred to the filter with alkali ( see Chapter 1 ) . Neutralize the filters ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml