Molecular Cloning: A Laboratory Manual, Book 2 |
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Page viii
... BACTERIOPHAGE VECTORS 2.55 Restriction and Modification 2.55 Amber Suppressors 2.55 Recombination Systems 2.55 Bacteriophage λ Growth , Purification , and DNA Extraction 2.60 PLAQUE PURIFICATION OF BACTERIOPHAGE X 2.60 Preparation of ...
... BACTERIOPHAGE VECTORS 2.55 Restriction and Modification 2.55 Amber Suppressors 2.55 Recombination Systems 2.55 Bacteriophage λ Growth , Purification , and DNA Extraction 2.60 PLAQUE PURIFICATION OF BACTERIOPHAGE X 2.60 Preparation of ...
Page xi
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Plating Bacteriophage M13 4.22 Picking Bacteriophage M13 Plaques 4.24 PREPARING STOCKS OF BACTERIOPHAGE M13 FROM SINGLE PLAQUES 4.25 Liquid Cultures 4.25 PREPARATION OF BACTERIOPHAGE M13 ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Plating Bacteriophage M13 4.22 Picking Bacteriophage M13 Plaques 4.24 PREPARING STOCKS OF BACTERIOPHAGE M13 FROM SINGLE PLAQUES 4.25 Liquid Cultures 4.25 PREPARATION OF BACTERIOPHAGE M13 ...
Page 15-63
... bacteriophage M13 vector ( usually bacteriophage M13mp18 or M13mp19 ) . Prepare single - stranded template DNA from a plaque generated by the recombinant bacteriophage . Methods for cloning into bacteriophage M13 vectors and for ...
... bacteriophage M13 vector ( usually bacteriophage M13mp18 or M13mp19 ) . Prepare single - stranded template DNA from a plaque generated by the recombinant bacteriophage . Methods for cloning into bacteriophage M13 vectors and for ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml