Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 12-3
... bind specifically to the oligonucleotide probes ( Singh et al . 1988 ; Vinson et al . 1988 ) . The DNA - binding domains of these proteins include such structural motifs as zinc fingers ( Miller et al . 1985 ; for review , see Evans and ...
... bind specifically to the oligonucleotide probes ( Singh et al . 1988 ; Vinson et al . 1988 ) . The DNA - binding domains of these proteins include such structural motifs as zinc fingers ( Miller et al . 1985 ; for review , see Evans and ...
Page 12-30
... binding site ( Singh et al . 1988 ) . However , under these circumstances , the signal from a positive clone was often weak and was easily washed from the filter . The equilibrium associa- tion binding constants of sequence - specific ...
... binding site ( Singh et al . 1988 ) . However , under these circumstances , the signal from a positive clone was often weak and was easily washed from the filter . The equilibrium associa- tion binding constants of sequence - specific ...
Page 12-43
... binding domains in the protein transcription factor IIIA from Xenopus oocytes . EMBO J. 4 : 1609 . Modrich , P. and I.R. Lehman . 1970. En- zymatic joining of polynucleotides . IX . A simple and rapid assay of polynu- cleotide joining ...
... binding domains in the protein transcription factor IIIA from Xenopus oocytes . EMBO J. 4 : 1609 . Modrich , P. and I.R. Lehman . 1970. En- zymatic joining of polynucleotides . IX . A simple and rapid assay of polynu- cleotide joining ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector