Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 87
Page 10-54
... buffer , it is necessary to carry out the restriction endonuclease digestion and end - labeling in two separate steps . In this case , cleave the DNA in the appropriate restriction enzyme buffer , remove the restriction enzyme by ...
... buffer , it is necessary to carry out the restriction endonuclease digestion and end - labeling in two separate steps . In this case , cleave the DNA in the appropriate restriction enzyme buffer , remove the restriction enzyme by ...
Page 12-18
... buffer gently for a further 30 minutes at room temperature . If necessary , the filters may be removed from the buffer at this stage , wrapped in Saran Wrap , and stored for up to 24 hours at 4 ° C . 11. Using blunt - ended forceps ...
... buffer gently for a further 30 minutes at room temperature . If necessary , the filters may be removed from the buffer at this stage , wrapped in Saran Wrap , and stored for up to 24 hours at 4 ° C . 11. Using blunt - ended forceps ...
Page 12-19
... buffers below in the order given . Transfer the filters individually from one buffer to the next . Use 7.5 ml of each buffer for each 82 - mm filter and 15 ml for each 138 - mm filter . TNT + 0.1 % bovine serum albumin TNT + 0.1 ...
... buffers below in the order given . Transfer the filters individually from one buffer to the next . Use 7.5 ml of each buffer for each 82 - mm filter and 15 ml for each 138 - mm filter . TNT + 0.1 % bovine serum albumin TNT + 0.1 ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml