Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-7
... cDNA library is N = In ( 1 - P ) In ( 1-1 / n ) where N = the number of clones required , P = the probability desired ( usually 0.99 ) , and 1 / n = the fractional proportion of the total mRNA that is repre- sented by ... cDNA Libraries 8.7.
... cDNA library is N = In ( 1 - P ) In ( 1-1 / n ) where N = the number of clones required , P = the probability desired ( usually 0.99 ) , and 1 / n = the fractional proportion of the total mRNA that is repre- sented by ... cDNA Libraries 8.7.
Page 8-46
... cDNA libraries for clones of interest : • Nucleic acid hybridization • Immunological detection of specific antigens ... cDNA sequences , whereas antibodies will react only with a subset of these clones ( in some cases one in six at best ) ...
... cDNA libraries for clones of interest : • Nucleic acid hybridization • Immunological detection of specific antigens ... cDNA sequences , whereas antibodies will react only with a subset of these clones ( in some cases one in six at best ) ...
Page 8-53
... cDNA libraries in bacteriophage A vectors . The scheme is based on the procedures of Gubler and Hoffman ( 1983 ) and Huynh et al . ( 1985 ) and has become the standard procedure used to ... cDNA Libraries 8.53 Protocols for cDNA Cloning 8.
... cDNA libraries in bacteriophage A vectors . The scheme is based on the procedures of Gubler and Hoffman ( 1983 ) and Huynh et al . ( 1985 ) and has become the standard procedure used to ... cDNA Libraries 8.53 Protocols for cDNA Cloning 8.
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml