Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 81
Page 8-47
... carried out under stringent conditions . Partially homologous probes Partially homologous probes are used to detect cDNA clones that are related , but not identical , to the probe sequences . If neither antibody nor nucleic acid probes ...
... carried out under stringent conditions . Partially homologous probes Partially homologous probes are used to detect cDNA clones that are related , but not identical , to the probe sequences . If neither antibody nor nucleic acid probes ...
Page 11-45
... carried out at 15-25 ° C below the calculated melting temperature ( Tm ) of a perfect hybrid . However , as the length of the probe is decreased , the Tm is lowered to the point where it is often impractical to carry out hybridization ...
... carried out at 15-25 ° C below the calculated melting temperature ( Tm ) of a perfect hybrid . However , as the length of the probe is decreased , the Tm is lowered to the point where it is often impractical to carry out hybridization ...
Page 13-43
... carried out synchronously . Some laboratories carry out all stages of sequencing reactions - from annealing to chain termination - in microtiter plates . In our experience , this is not advisable . During annealing , when small volumes ...
... carried out synchronously . Some laboratories carry out all stages of sequencing reactions - from annealing to chain termination - in microtiter plates . In our experience , this is not advisable . During annealing , when small volumes ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml