Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 82
Page 8-37
... carry an intact cI gene is therefore suppressed on strains of E. coli that carry an hfl mutation . In contrast , the growth of recombinant bacteriophages that cannot synthesize an active cI gene product is not inhibited ( see Hoyt et al ...
... carry an intact cI gene is therefore suppressed on strains of E. coli that carry an hfl mutation . In contrast , the growth of recombinant bacteriophages that cannot synthesize an active cI gene product is not inhibited ( see Hoyt et al ...
Page 8-41
... carry the sequence pTT at their 5 ' termini will generate unmethylated HindIII sites at both their 5 ' and 3 ' termini . Neither class of cDNA can be cloned into AORF8 by the directional method . Finally , in comparison to Agt11 , AORF8 ...
... carry the sequence pTT at their 5 ' termini will generate unmethylated HindIII sites at both their 5 ' and 3 ' termini . Neither class of cDNA can be cloned into AORF8 by the directional method . Finally , in comparison to Agt11 , AORF8 ...
Page 15-44
... carry a kan ' gene and therefore a BglII linker in the target region . 3. From the numbers of colonies obtained and the proportion of clones with a BglII linker in the target region , determine which pilot ligation reaction ( s ) ...
... carry a kan ' gene and therefore a BglII linker in the target region . 3. From the numbers of colonies obtained and the proportion of clones with a BglII linker in the target region , determine which pilot ligation reaction ( s ) ...
Other editions - View all
Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml