Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-44
... carrying the DNA to a source of ultraviolet irradiation ( 254 nm ) . Caution : Ultraviolet radiation is dangerous , particularly to the eyes . To minimize exposure , make sure that the ultraviolet light source is adequately shielded and ...
... carrying the DNA to a source of ultraviolet irradiation ( 254 nm ) . Caution : Ultraviolet radiation is dangerous , particularly to the eyes . To minimize exposure , make sure that the ultraviolet light source is adequately shielded and ...
Page 12-30
... carrying immobi- lized proteins are screened with 32P - labeled double - stranded DNA rather than with antibodies . Initially , screening was carried out with radiolabeled double - stranded DNA carrying a single binding site ( Singh et ...
... carrying immobi- lized proteins are screened with 32P - labeled double - stranded DNA rather than with antibodies . Initially , screening was carried out with radiolabeled double - stranded DNA carrying a single binding site ( Singh et ...
Page 15-44
... carrying the kan ' gene 1. Ligate the linearized plasmid DNA ( step 6 , page 15.42 ) to the fragment carrying the kan ' gene ( step 7 , page 15.43 ) . Because the efficiency with which the two fragments will ligate to each other is ...
... carrying the kan ' gene 1. Ligate the linearized plasmid DNA ( step 6 , page 15.42 ) to the fragment carrying the kan ' gene ( step 7 , page 15.43 ) . Because the efficiency with which the two fragments will ligate to each other is ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml