Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 74
Page 9-16
... centrifuge tube . Recover the cells by centrifugation at 1500g for 10 minutes at 4 ° C . Resuspend the cells in 5-10 volumes of ice - cold TBS and repeat the centrifugation . Resuspend the cells in TE ( pH 8.0 ) at a concentration of 5 ...
... centrifuge tube . Recover the cells by centrifugation at 1500g for 10 minutes at 4 ° C . Resuspend the cells in 5-10 volumes of ice - cold TBS and repeat the centrifugation . Resuspend the cells in TE ( pH 8.0 ) at a concentration of 5 ...
Page 14-23
... centrifuge , and take off the retentate cup . Add 0.75 ml of H2O to the sample reservoir , replace the retentate cup , and recentrifuge as described in step 4 . 6. Repeat step 5 twice more . At the end of the fourth centrifugation , the ...
... centrifuge , and take off the retentate cup . Add 0.75 ml of H2O to the sample reservoir , replace the retentate cup , and recentrifuge as described in step 4 . 6. Repeat step 5 twice more . At the end of the fourth centrifugation , the ...
Page 15-41
... centrifugation at 12,000g for 10 minutes at 4 ° C in a microfuge . Carefully decant the ethanol , and wash the pellet of DNA with 300 μl of 70 % ethanol at room temperature . Again , recover the DNA by centrifugation , and remove the 70 ...
... centrifugation at 12,000g for 10 minutes at 4 ° C in a microfuge . Carefully decant the ethanol , and wash the pellet of DNA with 300 μl of 70 % ethanol at room temperature . Again , recover the DNA by centrifugation , and remove the 70 ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml