Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxxii
... CHROMATOGRAPHY E.30 GEL - FILTRATION CHROMATOGRAPHY E.35 Preparation of Sephadex E.35 Column Chromatography E.36 SPUN - COLUMN CHROMATOGRAPHY E.37 PREPARATION OF DIALYSIS TUBING E.39 Appendix F : Subcloning FILLING RECESSED 3 ' TERMINI ...
... CHROMATOGRAPHY E.30 GEL - FILTRATION CHROMATOGRAPHY E.35 Preparation of Sephadex E.35 Column Chromatography E.36 SPUN - COLUMN CHROMATOGRAPHY E.37 PREPARATION OF DIALYSIS TUBING E.39 Appendix F : Subcloning FILLING RECESSED 3 ' TERMINI ...
Page 11-29
... Chromatography on a Silica Gel Isolate oligonucleotides from the filtered solution ( step 15 , page 11.28 ) either by reversed - phase chromatography on a silica gel as described below or by precipitation with ethanol . Precipitation ...
... Chromatography on a Silica Gel Isolate oligonucleotides from the filtered solution ( step 15 , page 11.28 ) either by reversed - phase chromatography on a silica gel as described below or by precipitation with ethanol . Precipitation ...
Page 11-37
... chromatography as described in step 2 above . allow the column to run dry . If necessary , Synthetic Oligonucleotide Probes 11.37 Purification of Radiolabeled Oligonucleotides by Chromatography Through Bio- Gel P-60 11 37.
... chromatography as described in step 2 above . allow the column to run dry . If necessary , Synthetic Oligonucleotide Probes 11.37 Purification of Radiolabeled Oligonucleotides by Chromatography Through Bio- Gel P-60 11 37.
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector