Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 59
Page 8-24
... cleaved vector with a phosphatase before ligation to cDNA . If the double - stranded cDNA contains one or more recognition sites for the restriction enzyme , it will be cleaved and subsequently cloned as two or more DNA fragments ...
... cleaved vector with a phosphatase before ligation to cDNA . If the double - stranded cDNA contains one or more recognition sites for the restriction enzyme , it will be cleaved and subsequently cloned as two or more DNA fragments ...
Page 13-99
... cleaved at adenines carry too many ring - opened 7- methylguanines and are heterogeneous in charge . Sample not evenly distributed in sample well . Nested end - labeled cleavage products be- come long enough to fold into stable hair ...
... cleaved at adenines carry too many ring - opened 7- methylguanines and are heterogeneous in charge . Sample not evenly distributed in sample well . Nested end - labeled cleavage products be- come long enough to fold into stable hair ...
Page 15-7
... cleave DNA frequently . Enzymes that generate blunt ends ( FnuDII , AluI , RsaI , and Hae III ) are particularly useful ... cleaved more than once by frequent- ly cutting restriction enzymes . Despite their problems , both of the methods ...
... cleave DNA frequently . Enzymes that generate blunt ends ( FnuDII , AluI , RsaI , and Hae III ) are particularly useful ... cleaved more than once by frequent- ly cutting restriction enzymes . Despite their problems , both of the methods ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml