Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-19
... cloned into any restriction site in the polycloning site of the vector . However , custom - made oligonucleotides complementary to specific sequences within the cloned DNA can also be used to prepare probes that represent only a portion ...
... cloned into any restriction site in the polycloning site of the vector . However , custom - made oligonucleotides complementary to specific sequences within the cloned DNA can also be used to prepare probes that represent only a portion ...
Page 14-5
... cloning and analysis of DNA , including : • Generation of specific sequences of cloned double - stranded DNA for use as probes • Generation of probes specific for uncloned genes by selective amplification of particular segments of cDNA ...
... cloning and analysis of DNA , including : • Generation of specific sequences of cloned double - stranded DNA for use as probes • Generation of probes specific for uncloned genes by selective amplification of particular segments of cDNA ...
Page 15-109
... cloned DNase I - gener- ated fragments . Nucleic Acids Res . 9 : 3015 . Barany , F. 1985a . Two - codon insertion mutagenesis of plasmid genes by using single - stranded hexameric oligonu- cleotides . Proc . Natl . Acad . Sci . 82 ...
... cloned DNase I - gener- ated fragments . Nucleic Acids Res . 9 : 3015 . Barany , F. 1985a . Two - codon insertion mutagenesis of plasmid genes by using single - stranded hexameric oligonu- cleotides . Proc . Natl . Acad . Sci . 82 ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml