Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-2
... complete sequence of the human genome . Although this rapid rate of progress has been catalyzed by many theoretical and technical advances , none has been so informative as the ability to construct libraries of eukaryotic genomic DNA ...
... complete sequence of the human genome . Although this rapid rate of progress has been catalyzed by many theoretical and technical advances , none has been so informative as the ability to construct libraries of eukaryotic genomic DNA ...
Page 9-36
... complete transfer depends on the size of the fragments of DNA , the porosity of the gel , and the strength of the applied field . However , because even high - molecular - weight nucleic acids migrate relatively rapidly from the gel ...
... complete transfer depends on the size of the fragments of DNA , the porosity of the gel , and the strength of the applied field . However , because even high - molecular - weight nucleic acids migrate relatively rapidly from the gel ...
Page 15-50
... complete , stain the gel for 1 hour in 1 × TAE containing ethidium bromide ( 0.5 μg / ml ) ( see Chapter 6 , pages 6.15- 6.17 ) . 4. Determine the amount of topoisomerase I that is required for complete conversion of the control plasmid ...
... complete , stain the gel for 1 hour in 1 × TAE containing ethidium bromide ( 0.5 μg / ml ) ( see Chapter 6 , pages 6.15- 6.17 ) . 4. Determine the amount of topoisomerase I that is required for complete conversion of the control plasmid ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml