Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxi
... Constructed in Plasmids 12.8 Expression Libraries Constructed in Bacteriophage λ 12.10 Using Antibodies in Immunological Screening 12.11 Choosing the Antibody 12.11 Purification of Antisera 12.13 Methods Used to Detect Antibodies Bound ...
... Constructed in Plasmids 12.8 Expression Libraries Constructed in Bacteriophage λ 12.10 Using Antibodies in Immunological Screening 12.11 Choosing the Antibody 12.11 Purification of Antisera 12.13 Methods Used to Detect Antibodies Bound ...
Page 8-38
... constructed in Agt11 , like those constructed in Agt10 , can be screened with nucleic acid probes . However , libraries constructed in Agt10 are far better suited for this purpose . An efficient method is available to select against ...
... constructed in Agt11 , like those constructed in Agt10 , can be screened with nucleic acid probes . However , libraries constructed in Agt10 are far better suited for this purpose . An efficient method is available to select against ...
Page 9-2
... constructed a library from fragments of mouse DNA that had been enriched approximately tenfold for ẞ - globin sequences by preparative gel electrophoresis and re- versed - phase chromatography ( Hardies and Wells 1976 ) . The ...
... constructed a library from fragments of mouse DNA that had been enriched approximately tenfold for ẞ - globin sequences by preparative gel electrophoresis and re- versed - phase chromatography ( Hardies and Wells 1976 ) . The ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml