Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 97
Page 9-7
... construction of genomic DNA libraries is now carried out using more modern vectors ( e.g. , the EMBL series , 2001 , ADASH , and Charon 38-40 [ see Chapter 2 , pages 2.30–2.37 ] ) that can propagate up to approximately 24 kb of foreign ...
... construction of genomic DNA libraries is now carried out using more modern vectors ( e.g. , the EMBL series , 2001 , ADASH , and Charon 38-40 [ see Chapter 2 , pages 2.30–2.37 ] ) that can propagate up to approximately 24 kb of foreign ...
Page 9-14
... construction of genomic DNA libraries in bacteriophage λ . The successful construction of libraries in cosmids , however , requires larger DNA . Fragments of DNA with one sheared end and one restriction - enzyme- generated end compete ...
... construction of genomic DNA libraries in bacteriophage λ . The successful construction of libraries in cosmids , however , requires larger DNA . Fragments of DNA with one sheared end and one restriction - enzyme- generated end compete ...
Page 15-32
... Construction of a linker - scanning mutant . ( a ) The nucleotide sequence of the normal HSV tk gene between 60 and 110 residues upstream of the transcription initiation site is shown on the top and bottom lines . This sequence is ...
... Construction of a linker - scanning mutant . ( a ) The nucleotide sequence of the normal HSV tk gene between 60 and 110 residues upstream of the transcription initiation site is shown on the top and bottom lines . This sequence is ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
88 other sections not shown
Other editions - View all
Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector