Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 83
Page 9-49
... containing 0.5 % SDS and 100 μg / ml denatured , fragmented , salmon sperm DNA ) . 50 × Denhardt's reagent contains 5 g of Ficoll ( Type 400 , Pharmacia ) 5 g of polyvinylpyrrolidone , 5 g of bovine serum albumin ( Pentex Fraction V ) ...
... containing 0.5 % SDS and 100 μg / ml denatured , fragmented , salmon sperm DNA ) . 50 × Denhardt's reagent contains 5 g of Ficoll ( Type 400 , Pharmacia ) 5 g of polyvinylpyrrolidone , 5 g of bovine serum albumin ( Pentex Fraction V ) ...
Page 11-48
... containing either sodium or tetramethylammonium ions . Hybrids involving oligonucleotides 16 and 19 nucleotides in length melt over a smaller range of temperature in solvents containing TMACl than in solvents containing sodium salts ( 3 ...
... containing either sodium or tetramethylammonium ions . Hybrids involving oligonucleotides 16 and 19 nucleotides in length melt over a smaller range of temperature in solvents containing TMACl than in solvents containing sodium salts ( 3 ...
Page 12-21
... containing the appropriate antibiotic . Remove the filter from the plate , invert it , and replace it , numbered side up . 3. Apply the bacteria in a small volume of liquid ( < 0.5 ml containing up to 20,000 bacteria for a 138 - mm ...
... containing the appropriate antibiotic . Remove the filter from the plate , invert it , and replace it , numbered side up . 3. Apply the bacteria in a small volume of liquid ( < 0.5 ml containing up to 20,000 bacteria for a 138 - mm ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml