Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 12-38
Grow a 2 - ml culture of E. coli strain Y1089 to saturation in LB medium
containing 0.2 % maltose , 50 ug / ml ampicillin , and 10 mm MgCl2 . 3. Dilute 50
ul of the saturated culture with 2 ml of LB medium containing 10 mm MgCl2 .
Transfer four ...
Grow a 2 - ml culture of E. coli strain Y1089 to saturation in LB medium
containing 0.2 % maltose , 50 ug / ml ampicillin , and 10 mm MgCl2 . 3. Dilute 50
ul of the saturated culture with 2 ml of LB medium containing 10 mm MgCl2 .
Transfer four ...
Page 12-39
Add 50 ul of each culture to 4 ml of prewarmed ( 30 ° C ) LB medium containing
50 ug / ml ampicillin . Continue incubation at 30 ° C with vigorous agitation . 9.
Grow the cultures until the OD 600 = 0.45 ( ~ 3 hours of incubation ) . It is
essential ...
Add 50 ul of each culture to 4 ml of prewarmed ( 30 ° C ) LB medium containing
50 ug / ml ampicillin . Continue incubation at 30 ° C with vigorous agitation . 9.
Grow the cultures until the OD 600 = 0.45 ( ~ 3 hours of incubation ) . It is
essential ...
Page 15-76
Add 5 ml of a mid - log - phase culture of E . coli strain CJ236 ( dut ung F ' ) (
Kunkel et al . 1987 ) . Incubate the culture with vigorous shaking ( 300 cycles /
minute on a rotary shaker ) for 6 hours at 37°C . The bacteriophage suspension
used ...
Add 5 ml of a mid - log - phase culture of E . coli strain CJ236 ( dut ung F ' ) (
Kunkel et al . 1987 ) . Incubate the culture with vigorous shaking ( 300 cycles /
minute on a rotary shaker ) for 6 hours at 37°C . The bacteriophage suspension
used ...
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LibraryThing Review
User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
Contents
CONCENTRATING NUCLEIC ACIDS E | 8-10 |
SYNTHESIS OF THE FIRST STRAND OF DNA 8 | 8-11 |
MEASUREMENT OF RADIOACTIVITY IN NUCLEIC ACIDS E | 8-18 |
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Common terms and phrases
aliquots allow amount amplification antibody appropriate approximately bacteriophage bacteriophage T4 base binding buffer carried cDNA cells centrifugation Chapter chromatography cleaved clones colonies complete concentration constructed containing deletions denatured described detection determine digestion dNTPs double-stranded efficiency electrophoresis et al ethanol expression extraction Figure filters four fragment gene genomic DNA hybridization increase Incubate inserted interest isolated Klenow labeled length libraries ligation linear linkers membrane method minutes mixture molecules mRNA mutants nucleic acids nucleotides obtained oligonucleotide plaques plasmid DNA plate position possible precipitation prepared presence primer probe problem protein purified radioactivity radiolabeled reaction recombinant region remove restriction enzyme room temperature samples screening separate sequence single single-stranded solution step stored strand synthesis target DNA target sequence template termini transfer Tris tube units usually vector volume