Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 82
Page 9-45
... denaturation / transfer solution for at least 5 minutes . Using a clean scalpel blade , cut a corner from the membrane to match the corner cut from the gel . 4. Transfer the denatured DNA from the gel to the membrane by capillary action ...
... denaturation / transfer solution for at least 5 minutes . Using a clean scalpel blade , cut a corner from the membrane to match the corner cut from the gel . 4. Transfer the denatured DNA from the gel to the membrane by capillary action ...
Page 10-14
... denatured , closed circular DNA or denatured , linear , double - stranded DNA ( e.g. , restriction fragments purified by gel electrophoresis ; Feinberg and Vogelstein 1983 , 1984 ) . The purified DNA , mixed with a molar excess of ...
... denatured , closed circular DNA or denatured , linear , double - stranded DNA ( e.g. , restriction fragments purified by gel electrophoresis ; Feinberg and Vogelstein 1983 , 1984 ) . The purified DNA , mixed with a molar excess of ...
Page 13-71
... denatured plasmid DNA by centrifugation at 12,000g for 15 minutes at 4 ° C in a microfuge . Carefully remove the supernatant by gentle aspiration , and then add 1 ml of 70 % ethanol ( 4 ° C ) to the tube . Recentrifuge for a further 2 ...
... denatured plasmid DNA by centrifugation at 12,000g for 15 minutes at 4 ° C in a microfuge . Carefully remove the supernatant by gentle aspiration , and then add 1 ml of 70 % ethanol ( 4 ° C ) to the tube . Recentrifuge for a further 2 ...
Other editions - View all
Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml