Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-61
... detection of RNA - DNA complexes . Biochem . Biophys . Res . Commun . 12:98 . Olszewska , E. and K. Jones . 1988. Vac- uum blotting enhances nucleic acid transfer . Trends Genet . 4:92 . Orkin , S.H. , A.F. Markham , and H.H. Kazazian ...
... detection of RNA - DNA complexes . Biochem . Biophys . Res . Commun . 12:98 . Olszewska , E. and K. Jones . 1988. Vac- uum blotting enhances nucleic acid transfer . Trends Genet . 4:92 . Orkin , S.H. , A.F. Markham , and H.H. Kazazian ...
Page 12-14
... detect antibody bound to its antigen on nitrocellulose filters are : • 125I - labeled antibodies reactive with species ... detection are generally more sensitive and give lower backgrounds than radiochemical methods . In addition ...
... detect antibody bound to its antigen on nitrocellulose filters are : • 125I - labeled antibodies reactive with species ... detection are generally more sensitive and give lower backgrounds than radiochemical methods . In addition ...
Page 12-41
... detection of alkaline phosphatase - conjugated anti- antibody on Western blots . Anal . Biochem . 136 : 175 . Bodner , M. , J.-L. Castrillo , L.E. Theill , T. Deerinck , M. Ellisman , and M. Karin . 1988. The pituitary - specific ...
... detection of alkaline phosphatase - conjugated anti- antibody on Western blots . Anal . Biochem . 136 : 175 . Bodner , M. , J.-L. Castrillo , L.E. Theill , T. Deerinck , M. Ellisman , and M. Karin . 1988. The pituitary - specific ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml