Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 75
Page 8-61
... determine empiri- cally the amount of reverse transcriptase that results in the highest yields and longest cDNAs ... determined by precipitation with ice - cold TCA ( see Appendix E ) , and its size should be determined by ...
... determine empiri- cally the amount of reverse transcriptase that results in the highest yields and longest cDNAs ... determined by precipitation with ice - cold TCA ( see Appendix E ) , and its size should be determined by ...
Page 8-73
... determine the amount of cDNA required to yield a population of bac- teriophages that contains 5 % recombinants . In this case , the cDNA is titrated to determine the amount that yields a tenfold stimulation of total plaques over the ...
... determine the amount of cDNA required to yield a population of bac- teriophages that contains 5 % recombinants . In this case , the cDNA is titrated to determine the amount that yields a tenfold stimulation of total plaques over the ...
Page 15-30
... determined in the exonuclease resistance assay ( Modrich and Lehman 1970 ) and to 60 cohesive - end units ( as defined by New England ... Determine the exact endpoints of the deletion in each 15.30 Site - directed Mutagenesis of Cloned DNA.
... determined in the exonuclease resistance assay ( Modrich and Lehman 1970 ) and to 60 cohesive - end units ( as defined by New England ... Determine the exact endpoints of the deletion in each 15.30 Site - directed Mutagenesis of Cloned DNA.
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml