Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-61
... determine empiri- cally the amount of reverse transcriptase that results in the highest yields and longest cDNAs ... determined by electrophoresis through alkaline agarose gels ( see Chapter 6 , pages 6.20-6.21 ) . For large - scale ...
... determine empiri- cally the amount of reverse transcriptase that results in the highest yields and longest cDNAs ... determined by electrophoresis through alkaline agarose gels ( see Chapter 6 , pages 6.20-6.21 ) . For large - scale ...
Page 11-55
A Laboratory Manual Joseph Sambrook, Tom Maniatis. EMPIRICAL DETERMINATION OF MELTING TEMPERATURE The melting temperature ( Tm ) of an oligonucleotide hybridized to a target sequence can be determined by the procedure described below ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. EMPIRICAL DETERMINATION OF MELTING TEMPERATURE The melting temperature ( Tm ) of an oligonucleotide hybridized to a target sequence can be determined by the procedure described below ...
Page 13-9
... determining the sequences of long tracts of DNA because of their very high processivity , their high rate of ... determined from a single set of reactions . In fact , the amount of sequence is limited more by the resolving power ...
... determining the sequences of long tracts of DNA because of their very high processivity , their high rate of ... determined from a single set of reactions . In fact , the amount of sequence is limited more by the resolving power ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml