Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 77
Page 9-24
... digestion of linkers ) to generate cohesive termini compatible with those of the vectors used to generate genomic DNA libraries ( Maniatis et al . 1978 ) . On the other hand , partial digestion with restriction enzymes that recognize ...
... digestion of linkers ) to generate cohesive termini compatible with those of the vectors used to generate genomic DNA libraries ( Maniatis et al . 1978 ) . On the other hand , partial digestion with restriction enzymes that recognize ...
Page 9-32
... digestion of high - molecular - weight DNA is unevenness of digestion caused by variations in the local concentrations of DNA . Clumps of DNA are relatively inaccessible to restriction enzymes and can be digested only from the outside ...
... digestion of high - molecular - weight DNA is unevenness of digestion caused by variations in the local concentrations of DNA . Clumps of DNA are relatively inaccessible to restriction enzymes and can be digested only from the outside ...
Page 13-21
... digestion with restriction en- zymes , sonication , and digestion with pancreatic DNAase I in the presence of Mn ** . Sonication and digestion with DNAase I are superior to digestion with restriction enzymes in a number respects . First ...
... digestion with restriction en- zymes , sonication , and digestion with pancreatic DNAase I in the presence of Mn ** . Sonication and digestion with DNAase I are superior to digestion with restriction enzymes in a number respects . First ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector