Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-8
... dilution of a small quantity of a stock solution of pancreatic DNAase I ( 1 mg / ml ) in ice - cold 1 × nick ... diluted DNAase I ( 10 ng / ml ) to the reaction . Mix by vortexing . 4. Add 2.5 units of E. coli DNA polymerase I ...
... dilution of a small quantity of a stock solution of pancreatic DNAase I ( 1 mg / ml ) in ice - cold 1 × nick ... diluted DNAase I ( 10 ng / ml ) to the reaction . Mix by vortexing . 4. Add 2.5 units of E. coli DNA polymerase I ...
Page 12-13
... diluted antiserum can be mixed with an extract of E. coli made by freeze - thawing and sonication . The absorbed diluted antiserum can then be used to probe expression libraries without further treatment . The most effective procedure ...
... diluted antiserum can be mixed with an extract of E. coli made by freeze - thawing and sonication . The absorbed diluted antiserum can then be used to probe expression libraries without further treatment . The most effective procedure ...
Page 13-75
... diluted stock solutions of ddNTPs and the 0.5 mm stock solutions of dNTPs ( see page 13.44 ) to make up chain - extension / chain - termination mixtures as shown below . Dispense the ddITP chain - extension / chain - termination ...
... diluted stock solutions of ddNTPs and the 0.5 mm stock solutions of dNTPs ( see page 13.44 ) to make up chain - extension / chain - termination mixtures as shown below . Dispense the ddITP chain - extension / chain - termination ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml