Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-21
... tailing can be recovered intact by cleavage with SalI and SphI . Plasmid Pstl mRNA AA Synthesize the first strand of cDNA Construction and Analysis of cDNA Libraries 8.21 MOLECULAR CLONING OF DOUBLE-STRANDED cDNA 8 Homopolymeric Tailing 8.
... tailing can be recovered intact by cleavage with SalI and SphI . Plasmid Pstl mRNA AA Synthesize the first strand of cDNA Construction and Analysis of cDNA Libraries 8.21 MOLECULAR CLONING OF DOUBLE-STRANDED cDNA 8 Homopolymeric Tailing 8.
Page 10-18
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Probes Single - stranded probes ... double - stranded probes : The absence of a com- plementary strand eliminates the possibility of forming nonproductive ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Probes Single - stranded probes ... double - stranded probes : The absence of a com- plementary strand eliminates the possibility of forming nonproductive ...
Page 15-20
... double- stranded DNA is given by the equation : dM t = -2VmMn dt [ Km + ( S ) , ] n = where M1 = molecular weight of linear double - stranded DNA after t minutes of digestion , Vm = maximum reaction velocity ( expressed as moles of nu ...
... double- stranded DNA is given by the equation : dM t = -2VmMn dt [ Km + ( S ) , ] n = where M1 = molecular weight of linear double - stranded DNA after t minutes of digestion , Vm = maximum reaction velocity ( expressed as moles of nu ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml