Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-18
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Probes Single ... DNA . For exam- ple , when using single - stranded DNA probes in nuclease ... double - stranded DNA templates into radiolabeled RNA ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Preparation of Single - stranded Probes Single ... DNA . For exam- ple , when using single - stranded DNA probes in nuclease ... double - stranded DNA templates into radiolabeled RNA ...
Page 14-28
... double - stranded DNA , which accumulates at an approximately exponential rate . After 12-15 cycles of amplification , the concentration of one of the primers becomes ... Single-stranded DNA Templates Generated Asymmetric Amplification 14 28.
... double - stranded DNA , which accumulates at an approximately exponential rate . After 12-15 cycles of amplification , the concentration of one of the primers becomes ... Single-stranded DNA Templates Generated Asymmetric Amplification 14 28.
Page 15-20
... double- stranded DNA is given by the equation : dM -2VM . = m n dt [ Km + ( S ) , ] m m where M1 = molecular weight of linear double - stranded DNA after t minutes of digestion , V = maximum reaction velocity ( expressed as moles of nu ...
... double- stranded DNA is given by the equation : dM -2VM . = m n dt [ Km + ( S ) , ] m m where M1 = molecular weight of linear double - stranded DNA after t minutes of digestion , V = maximum reaction velocity ( expressed as moles of nu ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector