Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xiii
... Electrophoresis 6.3 Factors Affecting the Rate of DNA Migration in Agarose Gels 6.4 MOLECULAR SIZE OF THE DNA 6.4 ... ELECTROPHORESIS BUFFER 6.6 Apparatuses Used for Agarose Gel Electrophoresis 6.8 PREPARATION AND EXAMINATION OF AGAROSE ...
... Electrophoresis 6.3 Factors Affecting the Rate of DNA Migration in Agarose Gels 6.4 MOLECULAR SIZE OF THE DNA 6.4 ... ELECTROPHORESIS BUFFER 6.6 Apparatuses Used for Agarose Gel Electrophoresis 6.8 PREPARATION AND EXAMINATION OF AGAROSE ...
Page xiv
... Electrophoresis 6.52 Preparation of DNA for Pulsed - field Gel Electrophoresis 6.53 ISOLATION OF INTACT DNA FROM MAMMALIAN CELLS 6.53 ISOLATION OF INTACT DNA FROM YEAST 6.53 RESTRICTION ENZYME DIGESTION OF DNA IN AGAROSE BLOCKS 6.57 ...
... Electrophoresis 6.52 Preparation of DNA for Pulsed - field Gel Electrophoresis 6.53 ISOLATION OF INTACT DNA FROM MAMMALIAN CELLS 6.53 ISOLATION OF INTACT DNA FROM YEAST 6.53 RESTRICTION ENZYME DIGESTION OF DNA IN AGAROSE BLOCKS 6.57 ...
Page 15-9
... electrophoresis through an agarose gel containing ethidium bromide ( 0.5 μg / ml ) , using as marker the plasmid DNA linearized in f . Electrophoresis should be continued until the linear DNA has migrated approximately one half the ...
... electrophoresis through an agarose gel containing ethidium bromide ( 0.5 μg / ml ) , using as marker the plasmid DNA linearized in f . Electrophoresis should be continued until the linear DNA has migrated approximately one half the ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml