Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 78
Page 8-55
... ethanol bath ) for 15 minutes or to store it for more prolonged periods at -20 ° C before centrifugation . In our hands , these procedures do not improve the efficiency with which DNA is precipitated by ethanol . • Save the ethanol ...
... ethanol bath ) for 15 minutes or to store it for more prolonged periods at -20 ° C before centrifugation . In our hands , these procedures do not improve the efficiency with which DNA is precipitated by ethanol . • Save the ethanol ...
Page 9-22
... ethanol at room temperature into each of a series of disposable 50 - ml polypropylene centrifuge tubes . Using wide - bore pi- pettes , carefully layer the cell suspensions under the ethanol . 5. Recover the DNA from each tube by slowly ...
... ethanol at room temperature into each of a series of disposable 50 - ml polypropylene centrifuge tubes . Using wide - bore pi- pettes , carefully layer the cell suspensions under the ethanol . 5. Recover the DNA from each tube by slowly ...
Page 13-98
... ethanol precipitation e . Poor suppression of Ts in the C ladder 2. Ts are weak in the C + T ladder . 3. Bands are present in C and / or C + T ladders at all guanine positions , not just those where guanine follows a pyrimi- dine in the ...
... ethanol precipitation e . Poor suppression of Ts in the C ladder 2. Ts are weak in the C + T ladder . 3. Bands are present in C and / or C + T ladders at all guanine positions , not just those where guanine follows a pyrimi- dine in the ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml