Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 32
Page vi
... Ethidium Bromide from DNAs Purified by Equilibrium Centrifugation in CsCl - Ethidium Bromide Gradients 1.46 METHOD 1 : EXTRACTION WITH ORGANIC SOLVENTS 1.46 METHOD 2 : ION - EXCHANGE CHROMATOGRAPHY 1.47 Decontamination of Ethidium ...
... Ethidium Bromide from DNAs Purified by Equilibrium Centrifugation in CsCl - Ethidium Bromide Gradients 1.46 METHOD 1 : EXTRACTION WITH ORGANIC SOLVENTS 1.46 METHOD 2 : ION - EXCHANGE CHROMATOGRAPHY 1.47 Decontamination of Ethidium ...
Page xiii
... ETHIDIUM BROMIDE 6.16 DECONTAMINATION OF DILUTE SOLUTIONS OF ETHIDIUM BROMIDE 6.17 Photography 6.19 Alkaline Agarose Gels 6.20 RECOVERY AND PURIFICATION OF DNA FRACTIONATED ON AGAROSE GELS 6.22 Electrophoresis onto DEAE - cellulose ...
... ETHIDIUM BROMIDE 6.16 DECONTAMINATION OF DILUTE SOLUTIONS OF ETHIDIUM BROMIDE 6.17 Photography 6.19 Alkaline Agarose Gels 6.20 RECOVERY AND PURIFICATION OF DNA FRACTIONATED ON AGAROSE GELS 6.22 Electrophoresis onto DEAE - cellulose ...
Page 9-33
... ethidium bromide ( ~ 200 ng ) should be applied to the gel . Occasionally , problems arise during loading of the gel because the DNA solution will not sink to the bottom of the well . This occurs when very - high - molecular - weight ...
... ethidium bromide ( ~ 200 ng ) should be applied to the gel . Occasionally , problems arise during loading of the gel because the DNA solution will not sink to the bottom of the well . This occurs when very - high - molecular - weight ...
Other editions - View all
Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml