Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxvii
... Expression in Mammalian Cells 16.68 Cloning by Expression of cDNA Clones 16.69 Expression Cloning of Genomic DNA 16.70 References 16.73 17 Expression of Cloned Genes in Escherichia coli Production of Fusion Proteins 17.3 Vector Systems ...
... Expression in Mammalian Cells 16.68 Cloning by Expression of cDNA Clones 16.69 Expression Cloning of Genomic DNA 16.70 References 16.73 17 Expression of Cloned Genes in Escherichia coli Production of Fusion Proteins 17.3 Vector Systems ...
Page 12-6
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Genomic DNA and cDNA Expression Libraries Two types of expression libraries can be constructed in plasmid and bac- teriophage expression vectors : cDNA and genomic DNA libraries . For ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Genomic DNA and cDNA Expression Libraries Two types of expression libraries can be constructed in plasmid and bac- teriophage expression vectors : cDNA and genomic DNA libraries . For ...
Page 12-8
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Expression Libraries Constructed in Plasmids Most cDNA expression libraries constructed in plasmid expression vectors use plasmids of the pUC , pUR , or pEX series ( see Chapters 1 and ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Expression Libraries Constructed in Plasmids Most cDNA expression libraries constructed in plasmid expression vectors use plasmids of the pUC , pUR , or pEX series ( see Chapters 1 and ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector