Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xiv
... Extraction with Strong Denaturants 7.18 EXTRACTION OF RNA WITH GUANIDINIUM THIOCYANATE FOLLOWED BY CENTRIFUGATION IN CESIUM CHLORIDE SOLUTIONS 7.19 EXTRACTION OF RNA WITH GUANIDINE HCI AND ORGANIC SOLVENTS 7.23 SELECTION OF POLY ( A ) ...
... Extraction with Strong Denaturants 7.18 EXTRACTION OF RNA WITH GUANIDINIUM THIOCYANATE FOLLOWED BY CENTRIFUGATION IN CESIUM CHLORIDE SOLUTIONS 7.19 EXTRACTION OF RNA WITH GUANIDINE HCI AND ORGANIC SOLVENTS 7.23 SELECTION OF POLY ( A ) ...
Page 9-16
... extraction buffer for each milliliter of cell suspension . Incubate the solution for 1 hour at 37 ° C , and then proceed to step 2 . Extraction buffer . 10 mM Tris Cl ( pH 8.0 ) 0.1 м EDTA ( pH 8.0 ) 20 μg / ml pancreatic RNAase 0.5 ...
... extraction buffer for each milliliter of cell suspension . Incubate the solution for 1 hour at 37 ° C , and then proceed to step 2 . Extraction buffer . 10 mM Tris Cl ( pH 8.0 ) 0.1 м EDTA ( pH 8.0 ) 20 μg / ml pancreatic RNAase 0.5 ...
Page 9-17
... extraction buffer ( see above ) in a beaker . Allow the powder to spread over the surface of the extraction buffer , and then shake the beaker to submerge the material . When all of the material is in solution , transfer the solution to ...
... extraction buffer ( see above ) in a beaker . Allow the powder to spread over the surface of the extraction buffer , and then shake the beaker to submerge the material . When all of the material is in solution , transfer the solution to ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml