Molecular Cloning: A Laboratory Manual, Book 2

activity addition aliquots allow amount amplification appropriate approximately bacteriophage bacteriophage T4 base buffer carried cDNA cells centrifugation Chapter chromatography cleavage cleaved clones complete concentration constructed containing deletions denatured described determine digestion dNTPs double-stranded efficiency electrophoresis et al ethanol expression extraction Figure filter four fragment gene genomic DNA hybridization increase Incubate inserted interest isolation Klenow labeled length libraries ligation linear linkers membrane method minutes mixture molecules mRNA mutations nucleic acids nucleotides obtained oligonucleotide original plasmid DNA plate position possible precipitation prepared presence primer probe problem protein purified radioactivity radiolabeled reaction recombinant region remove restriction enzyme room temperature samples screening separate sequence single single-stranded solution specific step stored strand synthesis target DNA target sequence template termini transfer Tris tube units usually vector volume