Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 87
Page 9-39
... filter in transfer buffer for at least 5 minutes . Using a clean scalpel blade , cut a corner from the nitrocellulose filter to match the corner cut from the gel . The rate at which different batches of nitrocellulose filters wet varies ...
... filter in transfer buffer for at least 5 minutes . Using a clean scalpel blade , cut a corner from the nitrocellulose filter to match the corner cut from the gel . The rate at which different batches of nitrocellulose filters wet varies ...
Page 12-17
... filters . Do not move the filter once contact is made with the plate . The easiest way to place the filter on the plate without trapping air bubbles is to hold it by its edges , bending it slightly so that the middle of the filter makes ...
... filters . Do not move the filter once contact is made with the plate . The easiest way to place the filter on the plate without trapping air bubbles is to hold it by its edges , bending it slightly so that the middle of the filter makes ...
Page 12-21
... filters ( Millipore HATF or equivalent ) ; replica filters are prepared by filter - to - filter contact . 1. Number the nitrocellulose filters with a soft - lead pencil or ballpoint pen , wet them with water , and sandwich them between ...
... filters ( Millipore HATF or equivalent ) ; replica filters are prepared by filter - to - filter contact . 1. Number the nitrocellulose filters with a soft - lead pencil or ballpoint pen , wet them with water , and sandwich them between ...
Other editions - View all
Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml