Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 12-18
... filters , chill the plates for 30 minutes at 4 ° C or 5 minutes at -20 ° C before peeling off the filters . To prepare duplicate filters : a . Remove the lids from the plates 30 minutes before removing the first filter . b . Chill the ...
... filters , chill the plates for 30 minutes at 4 ° C or 5 minutes at -20 ° C before peeling off the filters . To prepare duplicate filters : a . Remove the lids from the plates 30 minutes before removing the first filter . b . Chill the ...
Page 12-21
... filters ( Millipore HATF or equivalent ) ; replica filters are prepared by filter - to - filter contact . 1. Number the nitrocellulose filters with a soft - lead pencil or ballpoint pen , wet them with water , and sandwich them between ...
... filters ( Millipore HATF or equivalent ) ; replica filters are prepared by filter - to - filter contact . 1. Number the nitrocellulose filters with a soft - lead pencil or ballpoint pen , wet them with water , and sandwich them between ...
Page 12-34
... filters are prepared in exactly the same way as described on pages 12.16-12.17 , steps 1-8 . The filters are then processed and screened as described below . The following method was provided by S. McKnight . 1. Remove the ...
... filters are prepared in exactly the same way as described on pages 12.16-12.17 , steps 1-8 . The filters are then processed and screened as described below . The following method was provided by S. McKnight . 1. Remove the ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml