Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-7
... foreign DNA approximately 15-20 kb in length . The genomic DNA libraries constructed in these vectors were the source of most of the cloned segments of mam- malian genomic DNA isolated before 1983 , and some of these libraries are used ...
... foreign DNA approximately 15-20 kb in length . The genomic DNA libraries constructed in these vectors were the source of most of the cloned segments of mam- malian genomic DNA isolated before 1983 , and some of these libraries are used ...
Page 10-29
... foreign DNA sequences can usually be inserted by directional cloning no further than 30-40 nucleotides downstream from the position at which RNA synthesis is initiated in vitro . In many cases , the polycloning site also provides a ...
... foreign DNA sequences can usually be inserted by directional cloning no further than 30-40 nucleotides downstream from the position at which RNA synthesis is initiated in vitro . In many cases , the polycloning site also provides a ...
Page 15-60
... foreign DNA carried in the vector is sequenced to eliminate the possibility of adventitious mutations at ectopic sites . Restriction enzymes are used to recover the foreign DNA ( or a subfragment carrying the desired mutation ) from the ...
... foreign DNA carried in the vector is sequenced to eliminate the possibility of adventitious mutations at ectopic sites . Restriction enzymes are used to recover the foreign DNA ( or a subfragment carrying the desired mutation ) from the ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml