Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 77
Page 11-40
... FRAGMENT OF E. coli DNA POLYMERASE I In some cases ( e.g. , when using oligonucleotides as probes in Southern hybridizations ) , it is important to radiolabel to the highest possible specific activity . At best , phosphorylation results ...
... FRAGMENT OF E. coli DNA POLYMERASE I In some cases ( e.g. , when using oligonucleotides as probes in Southern hybridizations ) , it is important to radiolabel to the highest possible specific activity . At best , phosphorylation results ...
Page 13-78
... fragment of DNA and typically yields preparations of complementary strands that are only par- tially pure . More frequently , therefore , the radiolabeled DNA is digested with one or more restriction enzymes , and the double - stranded ...
... fragment of DNA and typically yields preparations of complementary strands that are only par- tially pure . More frequently , therefore , the radiolabeled DNA is digested with one or more restriction enzymes , and the double - stranded ...
Page 15-36
... fragment containing a kanamycin resistance gene ( kan ' ) . Because only those linear DNAs containing BglII sites at each end will incorporate the kan ' gene , all bacteria transformed to kanamycin resistance by the products of ligation ...
... fragment containing a kanamycin resistance gene ( kan ' ) . Because only those linear DNAs containing BglII sites at each end will incorporate the kan ' gene , all bacteria transformed to kanamycin resistance by the products of ligation ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector