Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-37
... gene product is controlled by the host hflA gene and the cIII gene product . Mutation of the hflA gene enhances the stability of the cII gene product , resulting in an increased production of the cI gene product and a high frequency of ...
... gene product is controlled by the host hflA gene and the cIII gene product . Mutation of the hflA gene enhances the stability of the cII gene product , resulting in an increased production of the cI gene product and a high frequency of ...
Page 11-14
... gene families occasionally display marked bias for or against certain codons ( e.g. , histone genes show a marked preference for codons enriched in A and T residues ) . If there is any evidence for such a bias in the gene of interest ...
... gene families occasionally display marked bias for or against certain codons ( e.g. , histone genes show a marked preference for codons enriched in A and T residues ) . If there is any evidence for such a bias in the gene of interest ...
Page 12-6
... gene of interest : the smaller the gene , the smaller the genomic DNA fragments . Ideally , the size of the fragments should be approximately one half the predicted size of the gene . This reduces the chance that the coding sequences of ...
... gene of interest : the smaller the gene , the smaller the genomic DNA fragments . Ideally , the size of the fragments should be approximately one half the predicted size of the gene . This reduces the chance that the coding sequences of ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector