Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-2
... genomic DNA libraries are now increasingly viewed as matrices that can be assembled into maps of the genome from which they were originally derived . The feasibility of using libraries in this way was established in the late 1970s ...
... genomic DNA libraries are now increasingly viewed as matrices that can be assembled into maps of the genome from which they were originally derived . The feasibility of using libraries in this way was established in the late 1970s ...
Page 11-7
... genome , are counted only once . Only approximate values of C are known for genomes that have not been sequenced completely . The following estimates are taken from Laird ( 1971 ) . Genome Complexitya Size a Escherichia coli 4.0 × 106 ...
... genome , are counted only once . Only approximate values of C are known for genomes that have not been sequenced completely . The following estimates are taken from Laird ( 1971 ) . Genome Complexitya Size a Escherichia coli 4.0 × 106 ...
Page 12-6
... genomic DNA expression library , the proportion of recombinants capable of expressing a fusion protein encoded by a gene whose coding region is 1.5 kb in length is predicted to be : Genome size ( kb ) Proportion E. coli Yeast 4.0 × 103 ...
... genomic DNA expression library , the proportion of recombinants capable of expressing a fusion protein encoded by a gene whose coding region is 1.5 kb in length is predicted to be : Genome size ( kb ) Proportion E. coli Yeast 4.0 × 103 ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml