Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-2
... DNA to the elucidation of the complete sequence of the human genome . Although this rapid rate of progress has been ... genomic DNA . Originally devised as banks from which individual bacterial genes could be isolated and analyzed ...
... DNA to the elucidation of the complete sequence of the human genome . Although this rapid rate of progress has been ... genomic DNA . Originally devised as banks from which individual bacterial genes could be isolated and analyzed ...
Page 9-7
... GENOMIC DNA The first generation of bacteriophage A vectors , such as Charon 4A ( see Chapter 2 , page 2.19 ) and Agt - WES ( see ... DNA 9.7 BACTERIOPHAGE À VECTORS RECOMMENDED FOR CONSTRUCTION LIBRARIES OF EUKARYOTIC GENOMIC DNA 9 7.
... GENOMIC DNA The first generation of bacteriophage A vectors , such as Charon 4A ( see Chapter 2 , page 2.19 ) and Agt - WES ( see ... DNA 9.7 BACTERIOPHAGE À VECTORS RECOMMENDED FOR CONSTRUCTION LIBRARIES OF EUKARYOTIC GENOMIC DNA 9 7.
Page 9-31
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Analysis of Genomic DNA by Southern Hybridization Localization of particular sequences within genomic DNA is ... GENOMIC DNA BY AGAROSE Analysis and Cloning of Eukaryotic Genomic DNA 9.31.
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Analysis of Genomic DNA by Southern Hybridization Localization of particular sequences within genomic DNA is ... GENOMIC DNA BY AGAROSE Analysis and Cloning of Eukaryotic Genomic DNA 9.31.
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml