Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 81
Page xiv
... ISOLATION OF INTACT DNA FROM YEAST 6.53 RESTRICTION ENZYME DIGESTION OF DNA IN AGAROSE BLOCKS 6.57 Markers for ... ISOLATION OF RNAS 7.6 Isolation of Total RNA from Mammalian Cells 7.6 Rapid Isolation of Total RNA from Mammalian Cells ...
... ISOLATION OF INTACT DNA FROM YEAST 6.53 RESTRICTION ENZYME DIGESTION OF DNA IN AGAROSE BLOCKS 6.57 Markers for ... ISOLATION OF RNAS 7.6 Isolation of Total RNA from Mammalian Cells 7.6 Rapid Isolation of Total RNA from Mammalian Cells ...
Page 8-9
... isolated by oligo ( dT ) chromatography . The immunoaffinity - purified mRNA , which represented only 0.01-0.05 % of the total mRNA , was used both to prepare cDNA probes and to construct cDNA clones . Using similar methods with ...
... isolated by oligo ( dT ) chromatography . The immunoaffinity - purified mRNA , which represented only 0.01-0.05 % of the total mRNA , was used both to prepare cDNA probes and to construct cDNA clones . Using similar methods with ...
Page 8-57
... isolated from a strain of E. coli lysogenic for a bacteriophage A that carries the gene ( polA ) that codes for the ... isolated from a strain of E. coli that expresses the RI restriction and modification genes from a multicopy plasmid ...
... isolated from a strain of E. coli lysogenic for a bacteriophage A that carries the gene ( polA ) that codes for the ... isolated from a strain of E. coli that expresses the RI restriction and modification genes from a multicopy plasmid ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml