Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-52
... labeled DNA fragments that can be used as size markers during gel electrophoresis . Because DNA fragments are labeled in proportion to their molar concen- trations and not their sizes , both small and large fragments in a restric- tion ...
... labeled DNA fragments that can be used as size markers during gel electrophoresis . Because DNA fragments are labeled in proportion to their molar concen- trations and not their sizes , both small and large fragments in a restric- tion ...
Page 13-78
... Labeling of Target DNA Double - stranded DNA can be labeled in the following ways : • At its 5 ' termini by bacteriophage T4 polynucleotide kinase and [ y - 32P ] ATP ( see Chapter 11 , pages 11.31-11.32 ) or at its 3 ' termini by calf ...
... Labeling of Target DNA Double - stranded DNA can be labeled in the following ways : • At its 5 ' termini by bacteriophage T4 polynucleotide kinase and [ y - 32P ] ATP ( see Chapter 11 , pages 11.31-11.32 ) or at its 3 ' termini by calf ...
Page 13-100
... labeled with polynucleotide kinase and [ y - P ] ATP ) . Probable causes Suggested solutions Reproduced with permission , from Maxam and Gilbert ( 1980. 11. An extraneous band across all four lad- ders at one position in a sequencing ...
... labeled with polynucleotide kinase and [ y - P ] ATP ) . Probable causes Suggested solutions Reproduced with permission , from Maxam and Gilbert ( 1980. 11. An extraneous band across all four lad- ders at one position in a sequencing ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml