Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-26
... length of the newly synthesized chains . Design the bacteriophage M13 or phagemid recombinant so that the probe will be as short as possible . If the average length of the DNA synthesized during the first stage of the reaction ( i.e. ...
... length of the newly synthesized chains . Design the bacteriophage M13 or phagemid recombinant so that the probe will be as short as possible . If the average length of the DNA synthesized during the first stage of the reaction ( i.e. ...
Page 11-8
... length ( which for screening of a typical mammalian cDNA library is 17-18 nucleotides [ Lathe 1985 ] ) , it is worthwhile increasing its length in order to maximize its specificity . The concomitant increase in mismatches that occurs as ...
... length ( which for screening of a typical mammalian cDNA library is 17-18 nucleotides [ Lathe 1985 ] ) , it is worthwhile increasing its length in order to maximize its specificity . The concomitant increase in mismatches that occurs as ...
Page 15-89
... length linear mole- cules . 5. Purify the full - length linear DNA by preparative agarose gel elec- trophoresis using one of the methods described in Chapter 6. Redissolve the DNA in TE ( pH 7.6 ) at a concentration of 250 μg / ml ...
... length linear mole- cules . 5. Purify the full - length linear DNA by preparative agarose gel elec- trophoresis using one of the methods described in Chapter 6. Redissolve the DNA in TE ( pH 7.6 ) at a concentration of 250 μg / ml ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector