Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 33
Page xxvii
... THAT CAN BE CLEAVED WITH FACTOR X 17.28 Expression of Secreted Foreign Proteins 17.31 phoA - MEDIATED EXPRESSION AND SECRETION 17.32 a QUANTITATING THE LEVELS OF EXPRESSION OF CLONED GENES 17.33 Monitoring Contents xxvii.
... THAT CAN BE CLEAVED WITH FACTOR X 17.28 Expression of Secreted Foreign Proteins 17.31 phoA - MEDIATED EXPRESSION AND SECRETION 17.32 a QUANTITATING THE LEVELS OF EXPRESSION OF CLONED GENES 17.33 Monitoring Contents xxvii.
Page 8-38
... levels of the lac repressor ( the product of the lacl gene ) , which prevents expression of the fusion protein during the initial stages of plaque formation . Thus , synthesis of potentially toxic products is arrested until the ...
... levels of the lac repressor ( the product of the lacl gene ) , which prevents expression of the fusion protein during the initial stages of plaque formation . Thus , synthesis of potentially toxic products is arrested until the ...
Page 14-4
... level of approximately 10 ° has been attained ) . If further amplification is required , a sample of the amplified DNA ... levels of 10 ° to 1010 can be achieved during the course of 60 sequential cycles of denaturation , annealing , and ...
... level of approximately 10 ° has been attained ) . If further amplification is required , a sample of the amplified DNA ... levels of 10 ° to 1010 can be achieved during the course of 60 sequential cycles of denaturation , annealing , and ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector